Journal of Dental Sciences
Volume 5, Issue 1 , Pages 30-35, March 2010

Enhancement of 5-aminolevulinic acid-induced photodynamic therapy by a bioadhesive polymer

  • Shu-Fang Chang

      Affiliations

    • Department of Dentistry, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
    • ,
  • Yu-Tsai Yang

      Affiliations

    • College of Oral Medicine, Taipei Medical University, Taipei, Taiwan
    • ,
  • Wei-Ling Li

      Affiliations

    • Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan
    • ,
  • Che-Tong Lin

      Affiliations

    • College of Oral Medicine, Taipei Medical University, Taipei, Taiwan
    • ,
  • Tsuimin Tsai

      Affiliations

    • College of Oral Medicine, Taipei Medical University, Taipei, Taiwan
    • Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan
    • Corresponding Author InformationCorresponding author. Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, 250, Wu-Hsing Street, Taipei 11042, Taiwan

Received 14 November 2009; accepted 20 February 2010.

Background/Purpose

Using a topical formulation of 5-aminolevulinic acid (ALA), a mediated photodynamic therapy (PDT) was developed to treat oral precancerous and cancerous lesions. Several clinical results demonstrated that this special ALA preparation containing Carbopol 971P (CP971P), a bioadhesive polymer, resulted in a satisfactory PDT effect. It is believed that this PDT efficacy may be due to the increased bioadhesion of the preparation at the treatment site. We investigated whether there was a beneficial effect of CP971P on ALA PDT in vitro.

Materials and methods

CP971P was co-incubated with ALA in a cell culture system. The effect of CP971P was evaluated by monitoring the uptake of ALA, the fluorescence intensity of protoporphyrin IX, and the cell survival rate.

Results

The polymer was found to enhance the fluorescence intensity of protoporphyrin IX and the subsequent PDT effect when concurrently dosed with ALA.

Conclusion

Our results showed that a formulation of ALA containing C971P could provide a better ALA PDT effect in cancer treatment.

Key Words:  aminolevulinic acid , Carbopol , photodynamic therapy , protoporphyrin IX

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PII: S1991-7902(10)60005-0

doi:10.1016/S1991-7902(10)60005-0

Journal of Dental Sciences
Volume 5, Issue 1 , Pages 30-35, March 2010

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