Effect of 1% chlorhexidine gel on the bonding strength to dentin
Article Outline
Background/Purpose
The purpose of this in vitro study was to evaluate the effect of 1% chlorhexidine (CHX) gel on dentin bond strengths of posterior composite resin applied with two different adhesive systems.
Material and methods
In total, 75 extracted, caries-free human molars were used. The occlusal surface of each tooth was ground to create a flat dentin surface. Then, each tooth was mounted in acrylic. The dentin specimens were randomly assigned to five groups of 15 specimens each. In Group 1, Prime & Bond NT (PBNT) was applied; in Group 2, a 1% CHX gel + etching for 15 s + PBNT were applied; in Group 3, etching + 1% CHX gel + PBNT were applied; in Group 4, Clearfil S3 Bond was applied; and in Group 5, 1% CHX gel + Clearfil S3 Bond were applied. A dentine bonding system was applied to dentin surfaces, and composite cylinders were built up using a special device and then light-polymerized. Specimens were mounted and sheared using an Instron universal testing machine at a cross-head speed of 0.5 mm/min. Results: The results were recorded in megapascals. The sheared specimens were examined under a light microscope, and the type of failure (adhesive, cohesive or mixed) was recorded. Data were compared by one-way analysis of variance and Tukey's honestly significant difference tests. Means were 16.4 ± 4.1 MPa in Group 1, 16.2 ± 3.9 MPa in Group 2, 13.0 ± 4.5 MPa in Group 3, 11.9 ± 2.7 MPa in Group 4, and 11.5 ± 2.7 MPa in Group 5. The use of 1% CHX gel before acid etching was significantly higher than after etching on the shear bond strength of PBNT (P < 0.05), but did not differ significantly from PBNT alone (P > 0.05).
Conclusion
Within the limitations of the present in vitro study, it was concluded that 1% CHX gel application did not adversely affect the shear bond strengths of dentin-bonding agents.
Key Words: adhesion , bond strength , cavity disinfection , chlorhexidine gel , posterior composite resin
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References
- . Current concepts of dental caries and its prevention . Oper Dent . 2001;26(Suppl 6):11–18
- . Clinical evaluation of two “packable” posterior composite resins two-year results . Clin Oral Investig . 2003;7:123–128
- . Cavity disinfectants and dentin bonding . Oper Dent . 1996;12:153–159
- . Effect of disinfectants on the bond strength of composite to dentin . Am J Dent . 1996;9:11–14
- . Bacterial inhibition produced by substances for dentin pre-treatment . Acta Odontol Latinoam . 1993;7:3–11
- . The cause of post-restorative sensitivity and its prevention . J Endod . 1986;12:475–481
- Chlorhexidine preserves dentin bond in vitro . J Dent Res . 2007;86:90–94
- . Effect of 2% chlorhexidine on microtensile bond strength of composite to dentin . J Adhes Dent . 2003;5:129–138
- . Dentinal tubule disinfection by chlorhexidine solutions: an in vitro study . Braz Endod J . 1997;2:55–57
- . Effect of chlorhexidine gel treatment supplemented with chlorhexidine varnish and resin on mutans streptococci and Actinomyces on root surfaces . Caries Res . 1990;24:242–247
- . In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond . Dent Mater . 2006;22:527–532
- . Chlorhexidine and related compounds . J Dent Res . 1989;68:1602–1608
- . Chlorhexidine varnishes: a review . J Clin Periodontol . 2002;29:1–8
- . Placement and replacement of resin-based composite restorations in Italy . Oper Dent . 1992;17:82–85
- . Response of the dental pulp to invasion of bacteria around three filling materials . J Dent Child . 1976;43:83–89
- . Shear bond strengths of composite to chlorhexidine-treated enamel . Am J Dent . 1994;7:85–88
- . Effect of chlorhexidine application on microtensile bond strength to dentin . Oper Dent . 2008;2:183–188
- . The effect of caries detector dyes and a cavity cleansing agent on composite resin bonding to enamel and dentin . J Clin Pediatr Dent . 2000;25:57–63
- . Bond strength to primary tooth dentin following disinfection with a chlorhexidine solution: an in vitro study . Pediatr Dent . 2003;25:49–52
- . Effect of disinfectant application methods on the bond strength of composite to dentin . J Oral Rehabil . 1999;26:836–940
- . Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo . J Dent Res . 2005;84:741–746
- . Dentin bonding: overview of the substrate with respect to adhesive material . J Esthet Dent . 1991;3:46–50
- Influence of chlorhexidine concentration on the durability of etch-and-rinse dentin bonds: a 12-month in vitro study . J Adhes Dent . 2009;11:191–198
- . Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine . Clin Diagn Lab Immunol . 1999;6:437–439
- . In vivo substantivity of 0.12% and 0.2% chlorhexidine mouthrinses on salivary bacteria . Clin Oral Investig . 2009;
- Effect of different cavity disinfectants on shear bond strength of composite resin to dentin . J Adhes Dent . 2009;11:343–346
- . Susceptibility of Streptococcus mutans and Streptococcus sobrinus to antimicrobial agents after short-term oral chlorhexidine treatment . J Oral Sci . 1995;103:32–35
- . Laboratory evaluation of one component enamel/dentin bonding agents . Am J Dent . 1996;9:206–210
- . Antimicrobial activity of cavity disinfectants . Gen Dent . 1999;47:187–190
- Adhesion to enamel and dentin: current status and future challenges . Oper Dent . 2003;28:215–235
PII: S1991-7902(10)60002-5
doi:10.1016/S1991-7902(10)60002-5
© 2010 Association for Dental Sciences of The Republic of China. Published by Elsevier Inc. All rights reserved.
