Journal of Dental Sciences
Volume 4, Issue 4 , Pages 178-186 , December 2009

Evaluation of cytotoxicity of resin bonding materials toward human oral epithelial cells using three assay systems

  • Ming-Gene Tu

      Affiliations

    • School of Dentistry, China Medical University, Taichung, Taiwan
    • Department of Dentistry, China Medical University Hospital, Taichung, Taiwan
    • ,
  • Wen-Miin Liang

      Affiliations

    • Institute of Environmental Health, China Medical University College of Public Health, Taichung, Taiwan
    • Biostatistics Center, China Medical University, Taichung, Taiwan
    • ,
  • Tai-Chin Wu

      Affiliations

    • Institute of Environmental Health, China Medical University College of Public Health, Taichung, Taiwan
    • Biostatistics Center, China Medical University, Taichung, Taiwan
    • ,
  • San-Yue Chen

      Affiliations

    • School of Dentistry, China Medical University, Taichung, Taiwan
    • Corresponding Author InformationCorresponding author. School of Dentistry, China Medical University, 91, Hsueh-Shih Road, Taichung 40402, Taiwan

Received 11 July 2009 ,Accepted 16 October 2009.

References 

  1. Hensten-Pettersen A , Jacobsen N . Toxic effects of dental materials . Int Dent J . 1991;41:265–273
  2. Ratanasathien S , Wataha JC , Hanks CT , Dennison JB . Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts . J Dent Res . 1995;74:1602–1606
  3. Lefebvre CA , Schuster GS . Biocompatibility of visible lightcured resin systems in prosthodontics . J Prosthet Dent . 1994;71:178–185
  4. Wiltshire WA , Ferreira MR , Ligthelm AJ . Allergies to dental materials . Quintessence Int . 1996;27:513–520
  5. Chigira H , Manabe A , Hasegawa T , et al.   Efficacy of various commercial dentin bonding systems . Dent Mater . 1994;10:363–368
  6. Chen RS , Liu CC , Tseng WY , Jeng JH , Lin CP . Cytotoxicity of three dentin bonding agents on human dental pulp cells . J Dent . 2003;31:223–229
  7. Ozen J , Atay A , Toksoy Topcu F , Ural AU , Dalkiz M , Tunca YM . Analysis of the cytotoxicity of four dentin bonding agents on gingival fibroblasts . Turk J Med Sci . 2005;35:395–399
  8. Schedle A , Franz A , Rausch-Fan X , et al.   Cytotoxic effects of dental composites, adhesive substances, compomers and cements . Dent Mater . 1998;14:429–440
  9. Caughman WF , Caughman GB , Dominy WT , Schuster GS . Glass ionomer and composite resin cements: effects on oral cells . J Prosthet Dent . 1990;63:513–521
  10. Vajrabhaya LO , Pasauk A , Harnirattisal C . Cytotoxicity evaluation of single component dentin bonding agent . Oper Dent . 2003;28:440–444
  11. Grobler SR , Olivier A , Moodley D , van W Kotze TW . Cytotoxicity of two concentrations of a dentin bonding agent on mouse 3T3 and human pulp fibroblast cell-lines . SADJ . 2004;59:368–372
  12. Yoshida K , Greener EH . Effects of two amine reducing agents on the degree of conversion and physical properties of an unfilled light-cured resin . Dent Mater . 1993;9:246–251
  13. Asmussen E , Peutzfeldt A . Influence of UEDMA BisGMA and TEGDMA on selected mechanical properties of experimental resin composites . Dent Mater . 1998;14:51–56
  14. Anseth KS , Goodner MD , Reil MA , Kannurpatti AR , Newman SM , Bowman CV . The influence of comonomer composition on dimethacrylate resin properties for dental composites . J Dent Res . 1996;75:1607–1612
  15. Johnson GH , Powell LV , Gordon GE . Dentin bonding systems: a review of current products and techniques . J Am Dent Assoc . 1991;122:34–41
  16. Full CA , Hollander WR . The composite resin restoration: a literature review, part I: proper cavity preparation and placement techniques . ASDC J Dent Child . 1993;60:48–51
  17. Schweikl H , Schmalz G , Rackebrandt K . The mutagenic activity of unpolymerized resin monomers in Salmonella typhimurium and V 79 cells . Mutat Res . 1998;451:119–130
  18. Kaga M , Noda M , Ferrracane JL , Nakamura W , Oguchi H , Sano H . The in vitro cytotoxicity of elutes from dentin bonding resins and their effect on tyrosine phosphorylation of L929 cells . Dent Mater . 2001;17:333–339
  19. Geurtsen W , Lehmann F , Spahl W , Leyhausen G . Cytotoxicity of 35 dental resin composite monomer/additives in permanent 3T3 and three human primary fibroblast culture . J Biomed Mater Res . 1998;41:474–480
  20. Issa Y , Watts DC , Brunton PA , Waters CM , Duxbury AJ . Resin composite monomers alter MTT and LDH activity of human gingival fibroblasts in vitro . Dent Mater . 2004;20:12–20
  21. Steele C , Leigh J , Swoboda R , Fidel PL . Growth inhibition of Candida by human oral epithelial cells . J Infect Dis . 2000;182:1479–1485
  22. Yamagata N , Oshima H . Cytotoxic effects of restorative materials on early passage cultured cells derived from human gingival . Shika Zairyo Kikai . 1990;9:541–554 [In Japanese, English abstract]
  23. Schweikl H , Schmalz G . Toxicity parameters for cytotoxicity testing of dental materials in two different mammalian cell lines . Eur J Oral Sci . 1996;104:292–299
  24. Livny  , Kaplan I , Reifen R , Polak-Charcon S , Madar Z , Schwartz B . Oral cancer cells differ from normal oral epithelial cells in tissue like organization and in response to lycopene treatment: an organotypic cell culture study . Nutr Cancer . 2003;47:195–209
  25. Njoroge T , Genco RJ , Sojar HT , Hamada N , Genco CA . A role for fimbriae in Porphyromonas gingivalis invasion of oral epithelial cells . Infect Immun . 1997;65:1980–1984
  26. Dorn BR , Leung KP , Fox AP . Invasion of human oral epithelial cells by Prevotella intermedia . Infect Immun . 1998;66:6054–6057
  27. Chen XS , Fang TL , Cai RB , Guo GL . Development of MTS/pms colorimetric assay in the proliferation of leukemic cells . Zhongguo Shi Yan Xue Ye Xue Za Zhi . 2002;10:438–440 [In Chinese, English abstract]
  28. Berridge MV , Tan AS , McCoy KA , Wang R . The biochemical and cellular basis of cell proliferation assays that use tetrazolium salts . Biochemica . 1996;4:15–19
  29. Crouch SPM , Kozlowski R , Slater KJ , Fletcher J . The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity . J Immunol Methods . 1993;160:81–88
  30. Maehara Y , Anai H , Tamada R , Sugimachi K . The ATP assay is more sensitive than the succinate dehydrogenase inhibition test for predicting cell viability . Eur J Cancer Clin Oncol . 1987;23:273–276
  31. Wataha JC , Lockwood PE , Bouillaguet S , Noda M . In vitro biological response to core and flowable dental restorative materials . Dent Mater . 2003;19:25–31
  32. Riberio DA , Duarte MAH , Matsumoto MA , Marque MEA , Salvadori DMF . Biocompatibility in vitro tests of mineral trioxide aggregate and regular and white Portland cements . J Endod . 2005;31:605–607
  33. Boland EJ , MacDougall M , Carnes DL , Dickens SH . In vitro cytotoxicity of a remineralizing resin-based calcium phosphate cement . Dent Mater . 2006;22:338–345
  34. Thonemann B , Schmalz G , Hiller KA , Schweikl H . Responses of L929 mouse fibroblasts, primary and immortalized bovine dental papilla-derived cell lines to dental resin components . Dent Mater . 2002;18:318–323
  35. Yoshii E . Cytotoxic effects of acrylates and methacrylates: relationships of monomer structures and cytotoxicity . J Biomed Mater Res . 1997;37:517–524
  36. Costa CA , Vaerten MA , Edwards CA , Hanks CT . Cytotoxic effects of current dental adhesive systems on immortalized odontoblast cell line MDPC-23 . Dent Mater . 1999;15:434–441
  37. Szep S , Kunkel A , Ronge K , Heidemann D . Cytotoxicity of modern dentin adhesives—in vitro testing on gingival fibroblasts . J Biomed Mater Res . 2002;63:53–60
  38. Huang FM , Chang YC . Cytotoxicity of dentin-bonding agents on human pulp cells in vitro . Int Endod J . 2002;35:905–909

PII: S1991-7902(09)60024-6

doi: 10.1016/S1991-7902(09)60024-6

Journal of Dental Sciences
Volume 4, Issue 4 , Pages 178-186 , December 2009

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