Journal of Dental Sciences
Volume 4, Issue 1 , Pages 13-17, March 2009

Upregulation of lysyl oxidase expression in cyclosporin A-induced gingival overgrowth

  • Chung-Hung Tsai

      Affiliations

    • Department of Pathology, Chung Shan Medical University Hospital, Taichung, Taiwan
    • Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan
    • ,
  • Tsai-Yu Chang

      Affiliations

    • Graduate School of Dentistry, Chung Shan Medical University, Taichung, Taiwan
    • ,
  • Yu-Chao Chang

      Affiliations

    • Graduate School of Dentistry, Chung Shan Medical University, Taichung, Taiwan
    • Department of Periodontics, Chung Shan Medical University Hospital, Taichung, Taiwan
    • Corresponding Author InformationCorresponding author. School of Dentistry, Chung Shan Medical University, No. 110, Chien-Kuo North Road, Section 1, Taichung 40201, Taiwan

Received 4 November 2008; accepted 24 January 2009.

Article Outline

Background/purpose

Lysyl oxidase (LOX) is involved in the initial steps of converting soluble monomers of collagen and elastin into insoluble fibers in the extracellular matrix. LOX was found to be upregulated in some fibrotic diseases. However, little is known about the correlation between LOX and cyclosporin A (CsA)-induced gingival overgrowth. The aim of this study was to compare LOX expression in normal healthy gingival tissues and CsA-induced gingival overgrowth specimens.

Materials and methods

Fifteen CsA-induced gingival overgrowth specimens and five normal gingival tissues were examined by immunohistochemistry. Three oral submucous fibrosis specimens were used as positive controls. In addition, one section from each CsA-induced gingival overgrowth specimen was stained with hematoxylin and eosin to evaluate the magnitude of inflammation at the histologic level. Differences in LOX expression between tissues with low and high levels of inflammation were subsequently analyzed using Fisher's exact test.

Results

LOX staining in gingival tissue was stronger in the CsA-induced gingival over-growth group than in the normal gingival group (P < 0.05). LOX staining was detected in the epithelium, connective tissue, inflammatory infiltrates, and endothelium. The LOX signal was mainly expressed in inflammatory cells (100%), followed by endothelial cells (93.3%), fibroblasts (80%) and epithelial cells (60%). In addition, LOX expression was significantly higher in CsA-induced gingival overgrowth specimens with higher levels of inflammatory infiltrates (P = 0.017).

Conclusion

LOX expression was significantly upregulated in CsA-induced gingival overgrowth specimens. In addition, the expression of LOX increased with the grade of inflammation in CsA-induced gingival overgrowth.

Key Words:  cyclosporin A , gingival overgrowth , lysyl oxidase

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PII: S1991-7902(09)60003-9

doi:10.1016/S1991-7902(09)60003-9

Journal of Dental Sciences
Volume 4, Issue 1 , Pages 13-17, March 2009

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