Journal of Dental Sciences
Volume 4, Issue 1 , Pages 7-12, March 2009

Concomitant upregulation of matrix metalloproteinase-2 in lesions and circulating plasma of oral lichen planus

  • Lo-Lin Tsai

      Affiliations

    • Institute of Stomatology, Chung Shan Medical University, Taichung, Taiwan
    • Oral Medicine Center, Chung Shan Medical University Hospital, Taichung, Taiwan
    • ,
  • Shun-Fa Yang

      Affiliations

    • Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan
    • ,
  • Chung-Hung Tsai

      Affiliations

    • Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan
    • Department of Oral Pathology, Chung Shan Medical University, Taichung, Taiwan
    • ,
  • Ming-Yung Chou

      Affiliations

    • Institute of Stomatology, Chung Shan Medical University, Taichung, Taiwan
    • Oral Medicine Center, Chung Shan Medical University Hospital, Taichung, Taiwan
    • ,
  • Yu-Chao Chang

      Affiliations

    • Institute of Stomatology, Chung Shan Medical University, Taichung, Taiwan
    • Oral Medicine Center, Chung Shan Medical University Hospital, Taichung, Taiwan
    • Corresponding Author InformationCorresponding author. Institute of Stomatology, College of Oral Medicine, Chung Shan Medical University, No. 110, Chien-Kuo North Road, Section 1, Taichung 40201, Taiwan

Received 17 November 2008; accepted 22 January 2009.

Article Outline

Background/purpose

Oral lichen planus (OLP) is a chronic inflammatory disorder characterized by a T cell-mediated immune response against epithelial cells. Matrix metalloproteinases (MMPs) are an important group of zinc enzymes and are thought to play an important role in the degradation of components of the extracellular matrix. The aim of this study was to assess the expression of MMP-2 and MMP-9 in both tissue specimens and circulating plasma.

Materials and methods

Twelve cases of OLP were collected. In addition, six cases with chronic inflammation and six normal subjects were also recruited. Oral tissue specimens were collected for measurement of MMPs by immunohistochemistry, and sera prepared from peripheral blood were used for gelatin zymography to determine MMP levels in plasma.

Results

The level of MMP-2 in patients with OLP was significantly higher than that in the other two groups, both in tissues and sera (P < 0.0001). However, there was no difference in the expression of MMP-9 among these groups.

Conclusion

MMP-2 overexpression in OLP is consistent with its upregulation in peripheral serum. This result also indicates that MMP-2 might play a role in the pathogenesis of OLP.

Key Words:  gelatin zymography , immunohistochemistry , matrix metalloproteinases , oral lichen planus , serum

No full text is available. To read the body of this article, please view the PDF online.

 

Back to Article Outline

References 

  1. Sugerman PB , Savage NW , Walsh LJ , Seymour GJ . Disease mechanisms in oral lichen planus: a possible role for autoimmunity . Aust J Dermatol . 1993;34:63–69
  2. Porter SR , Kirby A , Olsen I , Barrett W . Immunologic aspects of dermal and oral lichen planus: a review . Oral Surg Oral Med Oral Pathol Oral Radiol Endod . 1997;83:358–366
  3. de Moura Castro Jacques C , Cardozo Pereira AL , Cabral MG , Cardoso AS , Ramos-e-Silva M . Oral lichen planus, part I: Epidemiology, clinics, etiology, immunopathogeny, and diagnosis . Skinmed . 2003;2:342–347
  4. Seoane J , Romero MA , Varela-Centelles P , Diz-Dios P , Garcia-Pola MJ . Oral lichen planus: a clinical and morpho-metric study of oral lesions in relation to clinical presentation . Braz Dent J . 2004;15:9–12
  5. Sugerman PB , Savage NW , Walsh LJ . The pathogenesis of oral lichen planus . Crit Rev Oral Biol Med . 2002;13:350–365
  6. Karagouni EE , Dotsika EN , Sklavounou A . Alteration in peripheral blood mononuclear cell function and serum cytokines in oral lichen planus . J Oral Pathol Med . 1994;23:28–35
  7. Goetzl EJ , Banda MJ , Leppert D . Matrix metalloproteinases in immunity . J Immunol . 1996;156:1–4
  8. Tsai CH , Hsieh YS , Yang SF , Chou MY , Chang YC . Matrix met-alloproteinase 2 and matrix metalloproteinase 9 expression in human oral squamous cell carcinoma and the effect of protein kinase C inhibitors: preliminary observations . Oral Surg Oral Med Oral Pathol Oral Radiol Endod . 2003;95:710–716
  9. Jungell PKY , Malmstrom M . Basement membrane changes in oral lichen planus . Proc Finn Dent Soc . 1989;85:119–124
  10. Salo T , Liotta LA , Tryggvason K . Purification and characterization of a murine basement membrane collagen degrading enzyme secreted by metastatic tumor cells . J Biol Chem . 1983;258:3058–3063
  11. Zhou XJ , Sugerman PB , Savage NW , Walsh LJ . Matrix metal-loproteinases and their inhibitors in oral lichen planus . J Cutan Pathol . 2001;28:72–82
  12. Krutchkoff DJ , Cutler L , Laskowski S . Oral lichen planus: the evidence regarding potential malignant transformation . J Oral Pathol . 1978;7:1–7
  13. Chang YC , Yang SF , Hsieh YS . Regulation of matrix metallo-proteinases-2 production by cytokines and pharmacological agents in human pulp cell cultures . J Endod . 2001;27:679–682
  14. Giannelli G , Brassard J , Foti C . Altered expression of basement membrane proteins and their integrin receptors in lichen planus: possible pathogenetic role of gelatinases A and B . Lab Invest . 1996;74:1091–1104
  15. Liu Z , Li N , Diaz LA . Synergy between a plasminogen cascade and MMP-9 in autoimmune disease . J Clin Invest . 2005;115:825–828
  16. Rhodus NL , Cheng B , Myers S , Bowles W , Ho V , Ondrey F . A comparison of the pro-inflammatory NF-kB-dependent cytokines: TNF-alpha, IL-1-alpha, IL-6, and IL-8 in different oral fluids from oral lichen planus patients . Clin Immunol . 2005;114:278–283
  17. Moon SK , Cha BY , Kim CH . ERK1/2 mediates TNF-a-induced matrix metalloproteinase-9 expression in human vascular smooth muscle cells via the regulation of NF-kb and AP-1: involvement of the Ras dependent pathway . J Cell Physiol . 2004;198:417–427
  18. Rajavashisth TB , Xu XP , Jovinge S , Meisel S , Xu XO , Chai NN . Membrane type 1 matrix metalloproteinase expression in human atherosclerotic plaques: evidence for activation by proinflammatory mediators . Circulation . 1999;99:3103–3109
  19. Kim SG , Chae CH , Cho BO , et al.   Apoptosis of oral epithelial cells in oral lichen planus caused by upregulation of BMP-4 . J Oral Pathol Med . 2006;35:37–45

PII: S1991-7902(09)60002-7

doi:10.1016/S1991-7902(09)60002-7

Journal of Dental Sciences
Volume 4, Issue 1 , Pages 7-12, March 2009

Article Tools